Competent Cells
MilliporeSigma™ Novagen™ Rosetta-gami™ 2 (DE3) Competent Cells
Rosetta-Gami 2 host strains allows for enhanced disulfide bond formation and enhanced expression of eukaryotic proteins that contain codons rarely used in E. coli.
MilliporeSigma™ Novagen™ Origami™ B Competent Cell Set
Origami B host strains carry the same trxB/gor mutations as the original Origami strains, except that they are derived from a lacZY mutant of BL21.
MilliporeSigma™ Novagen™ BL21 (DE3) pLysS Singles™ Competent Cells
BL21 strains are the most widely used hosts for protein expression from pET recombinants and have the advantage of being deficient in both lon and ompT proteases.
MilliporeSigma™ Novagen™ Tuner™ (DE3) pLacI Competent Cells
Tuner host strains are lacZY deletion mutants of BL21, which enable adjustable levels of protein expression throughout all cells in a culture. Contains the pLacI plasmid producing extra Lac repressor.
MilliporeSigma™ Novagen™ Rosetta-gami™ B (DE3) Competent Cells
Rosetta-gami B strains combine the key features of BL21, Origami, and Rosetta to enhance both the expression of eukaryotic proteins and the formation of target protein disulfide bonds.
MilliporeSigma™ Novagen™ Rosetta™ 2 (DE3) Singles Competent Cells
Novagen's Rosetta™ 2 host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. Coli.
MilliporeSigma™ Novagen™ Tuner™ Competent Cell Set
Tuner™ strains are lacZY deletion mutants of BL21 and enable adjustable levels of protein expression throughout all cells in a culture.
MilliporeSigma™ Novagen™ BL21(DE3) pLysS Competent Cells
BL21 host strain that expresses T7 RNA polymerase and also encode T7 lysozyme that suppresses basal expression of toxic target proteins prior to induction.
MilliporeSigma™ Novagen™ Origami™ B (DE3) Competent Cells
Origami B host strains carry the same mutations as the original Origami strain, except that they are derived from a lacZY mutant of BL21 to enable precise control of expression levels using IPTG.
MilliporeSigma™ Novagen™ Rosetta™ 2(DE3)pLacI Competent Cells
Rosetta strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. Contains the pLacI plasmid producing extra Lac repressor.
MilliporeSigma™ Novagen™ Tuner™ DE3 Competent Cells
Tuner host strains are lacZY deletion mutants of BL21, which enable adjustable levels of protein expression throughout all cells in a culture.
MilliporeSigma™ Novagen™ Origami™ 2 (DE3) pLysS Competent Cells
Origami 2 strains have mutations in glutathione reducatase(gor) and thioredoxin reductase (trxB), facilitating proper disulfide bond formation. T7 lysozyme expression suppresses basal T7 expression.
MilliporeSigma™ Novagen™ HMS174 Competent Cells
HMS174 strains provide high transformation efficiencies and the recA mutation in a K-12 background. Strain may stabilize certain target genes whose products may cause the loss of the DE3 prophage.
MilliporeSigma™ Novagen™ NovaBlue Competent Cells
NovaBlue is a K-12 strain ideally suited as an initial cloning host due to its high transformation efficiency, blue/white screening capability (with appropriate plasmids) and recA endA mutations.
MilliporeSigma™ Novagen™ Origami™ 2 (DE3) Competent Cells
Origami 2 strains have mutations in glutathione reducatase (gor) and thioredoxin reductase (trxB), facilitating proper disulfide bond formation.